Abstract:
The study was conducted to determine the components of Ipil-ipil (LeucaenaLeococephala) stem that has anti-bacterial property based on the phytochemical analysis. It aimed to: (1) analyse the bacterial effect of Ipil-ipil stem extract and the control antibiotic, amoxicillin, against S. and, (2) find the best concentration to inhibit the growth of the S. aureus bacteria.
The experimental quantitative research design was employed specifically; it utilized the decoction method to obtain the extracts. This was carried at the College of Veterinary Medicine laboratory in BSU from February 23-26, 2016.
To treat the data, Analysis of Variances (ANOVA) and Duncan’s Multiple Range Test (DMRT) were used. Five treatments were prepared: four concentrations of ipil-ipil stem extract; and the control –amoxicillin. Treatment 1 comprised of 100% ipil-ipil stem extract and 0% distilled water; Treatment 2 with of 75% ipil-ipil stem extract and 25% distilled water, Treatment 3 contained 50% ipil-ipil stem extract and 50% distilled water; and Treatment 4 comprised of 25% ipil-ipil stem extract and 75% distilled water. Each treatment had three replicates. Amoxicillin, on the other hand, was dissolved in a 10 mL distilled water. Notably, the zones of inhibition of the bacteria were measured using a verniercaliper.
Findings revealed that terpenes, phenolics, saponins, and flavonoids are present in the ipil-ipil stem extract which have antibacterial properties. The presence these indicate various ways in which the stem may be used. Primarily, it may benefit the community for its antibacterial properties; as per related literature may affect the increase of zone of inhibition of S. aureus.
Based on the results, the following recommendations were identified: studies may be conducted to confirm as to potentials the antibacterial components of ipil-ipil stem like terpenes, phenolics, and flavonoids; upcoming researchers may use other extraction techniques such as ethanol and crude methods; maximize the other parts of the plant as testing potentials of the roots and flowers inhibiting the growth of S. aureus; and, future studies anchored on the research direction may be conducted imploring on the other means by which the ipil-ipil stem extract may be utilized.
