Abstract:
This study was conducted to determine the effect of different treatments of malunggay leaf extract and the control treatment, ampicillin, on Escherichia coli and to determine the most effective concentration of malunggay leaf extract as compared to the control (ampicillin) as treatment against Escherichia coli.
The researchers used the Completely Randomized Design (CRD), also called the Analysis of Variance (ANNOVA) and the Duncan’s Multiple Range Test (DMRT). Four (4) treatments were used in total including the control, ampicillin, and the 75%, 50% and 25%malunggayleaf extract concentrations. Treatment 1 was comprised of 75% malunggay leaves extract and 25% ml distilled water. Treatment 2 was composed of 50% malunggay leaf extractand 50% ml of distilled water. Treatment 3 was composed of 25% malunggay leaves extract and 75% distilled water. Three (3) trials were prepared for each treatment. Each trial had three (3) replicates. The study used ampicillin as control treatment. The zone of inhibition of the bacteria was measured using a ruler.
Findings showed that all concentrations of malunggay leaf extract used were not effective in inhibiting the growth of Escherichia coli as compared with the control agent, ampicillin.
From the data gathered, analyzed and interpreted, the following recommendations are forwarded. Based on the findings and conclusions, the following are recommended: to lower the concentration of the malunggay extract to determine its effect against Escherichia coli. Other parts of the malunggay plant should be used to determine its antibacterial effects on Escherichia coli. The use of other forms of extraction of the malunggay should be explored to measure the efficacy against Escherichia coli. To determine the efficacy of malunggay extract on the other forms of bacteria. The same study be conducted to validate the above findings.
