Artificial Production of Dojo Loach (Misgurnus anguillicaudatus) Using Ovaprim® Injectable Solution

Abstract

The study was conducted to produce fry through artificial production using Ovaprim® (Salmon Gonadotropin Releasing Hormone Analog 20 μg/ml + Domperidone 10 mg/ml). Brood stock were collected from Bauko, Mountain Province and Atok, Benguet. They were injected with Ovaprim® to induce egg and sperm maturation after which, the ova were collected by stripping while the sperm were collected by sacrificing the male and collecting the testes. Artificial fertilization was done and incubation took place in improvised hatching trays. Thirty hours after fertilization, counting was done and embryo development was observed and noted at 6, 10, 25, 30, and 45 hours after fertilization. The ratio of male breeders and female breeders used in the study were 1 male to 2 females. Results showed that out of the 18 females used, about 13,432 eggs were collected, from which 2,555 eggs hatched at 30 hours after fertilization. After the yolk sac had disappeared in the larvae (fry), these were fed with hard-boiled egg yolk. The larvae survived only until the fourth day. The suspected cause of mortality was a ciliated protozoan parasite, Chilodonella sp., which increased in population when water condition and the fishes’ condition favoured their growth. Further studies need to be conducted with regards to water quality and larvae culture to increase the survival rate, as well the efficacy of other hormone preparations recommended for use in food fish.